Endophytic fungi harbored in : diversity and potential as biological control agents against host plant pathogens of root-rot disease.

Background: Endophytic fungi play an important role in balancing the ecosystem and boosting host growth. In the present study, we investigated the endophytic fungal diversity of healthy and evaluated its potential antimicrobial activity against five major phytopathogens causing root-rot of .

Methods: A culture-dependent technique, combining morphological and molecular methods, was used to analyze endophytic fungal diversity. A double-layer agar technique was used to challenge the phytopathogens of .

Results: A total of 89 fungi were obtained from the roots, stems, leaves, and seeds of , and 41 isolates representing different morphotypes were selected for taxonomic characterization. The fungal isolates belonged to Ascomycota (96.6%) and Zygomycota (3.4%). All isolates were classified to 23 genera and an unknown taxon belonging to Sordariomycetes. The number of isolates obtained from different tissues ranged from 12 to 42 for leaves and roots, respectively. The selected endophytic fungal isolates were challenged by the root-rot pathogens , , , , and Twenty-six of the 41 isolates (63.4%) exhibited activity against at least one of the pathogens tested.

Conclusion: Our results suggested that harbors diversified endophytic fungi that would provide a basis for the identification of new bioactive compounds, and for effective biocontrol of notoginseng root rot.