AI Article Synopsis
- UFP is a hybrid staining technique that improves quality and reduces time for cytological analysis, using a modified approach with Harris Hematoxylin.
- The study aimed to compare the effectiveness of the modified ultrafast Papanicolaou (MUFP) stain with traditional stains like PAP, H and E, and Giemsa in fine needle aspiration cytology (FNAC) of head and neck swellings.
- Results showed that MUFP provided significantly better quality smears in terms of background, cell morphology, nuclear staining, and overall pattern, making it a viable alternative for cytological studies.
Article Abstract
Introduction: Ultrafast Papanicolaou stain (UFP) was introduced as a hybrid of Romanowsky and Papanicolaou (PAP) stain. It enhances the quality and reduces the time. In the present study, a modified staining technique was adapted where Gill's Hematoxylin was replaced by Harris Hematoxylin.
Aims: The aim of the study was to assess the use of the modified ultrafast Papanicolaou (MUFP) stain for fine needle aspiration cytology (FNAC) of head and neck swellings in comparison with the routine PAP stain, hematoxylin and eosin (H and E), and Giemsa.
Materials And Methods: Forty FNACs of head and neck swellings were collected. FNAC procedure was performed by standard method; two smears were fixed in 95% propanol and stained with PAP and H and E. Two smears were air dried, 1 was stained with Giemsa, and 1 was rehydrated with normal saline, fixed in alcoholic formalin, and stained with MUFP. Four parameters were considered and scored background, cell morphology, nuclear staining, and overall staining pattern).
Results: The quality of MUFP smears were better when compared to routine PAP, H and E, and Giemsa, and was statistically significant by Wilcoxon matched pair test.
Conclusions: MUFP stain in comparison to routine PAP, H and E, and Giemsa provides an excellent and suitable alterative in cytological staining for the study of various organs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5492752 | PMC |
| http://dx.doi.org/10.4103/JOC.JOC_23_16 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Shorr Versus Modified Ultrafast Papanicolaou Method for Intraoperative Diagnosis of Peritoneal Washing Cytology in Advanced Gastric Cancer: A Phase II Study.
J Gastric Cancer
October 2023
Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Korea.
Purpose: According to the American Joint Committee on Cancer cancer staging system, positive peritoneal washing cytology (PWC) indicates stage IV gastric cancer. However, rapid intraoperative diagnosis of PWC has no established reliable method. This study evaluated and compared the diagnostic accuracy of the Shorr and the modified ultrafast Papanicolaou (MUFP) methods for intraoperative PWC.
View Article and Find Full Text PDFQualified sperm selection based on the rheotaxis and thigmotaxis in a microfluidic system.
Biomed Eng Lett
November 2023
Department of Chemical and Petroleum Engineering, Sharif University of Technology, 11155-9465, Tehran, Iran.
Unlabelled: Microfluidic systems with the ability to mimic the female reproductive tract (FRT) and sperm features have emerged as promising methods to separate sperm with higher quality for the assistant reproductive technology. Thereby, we designed and fabricated a microfluidic system based on FRT features with a focus on rheotaxis and thigmotaxis for passive sperm separation. In this regard, four various geometries (linear, square, zigzag, and sinusoidal) were designed, and the effect of rheotaxis and thigmotaxis were investigated.
View Article and Find Full Text PDFA New Cytology Staining Method: A Fast Approach for Rapid On-Site Evaluation on Thyroid Fine-Needle Aspiration Cytology.
Acta Cytol
June 2023
IPATIMUP, Institute of Molecular Pathology and Immunology of University of Porto, Porto, Portugal,
Introduction: Thyroid fine-needle aspiration (FNA) is a well-established technique in the cytology literature. Through the introduction of rapid stains in cytology practice, the ever-increasing utility of rapid on-site evaluation (ROSE) has strengthened the place of FNA as a primary diagnostic method in patient management. There are few stain variants available in the market for ROSE, namely Diff-Quik (DQ), Toluidine blue, and ultrafast Papanicolaou stains.
View Article and Find Full Text PDFEvaluation of Role of Intraoperative Cytology Technique in Diagnosis and Management of Cancer.
J Cytol
June 2020
Department of Pathology, Indira Gandhi Government Medical College, Nagpur, Maharashtra, India.
Context: Intraoperative pathological assessment provides valuable information in a patient's diagnosis and management. Touch imprint/crush cytology is simple, rapid, and cost-effective. Also, imprint smears give excellent cytomorphology.
View Article and Find Full Text PDFModified Ultrafast Papanicolaou Stain in Ultrasound Guided FNAC of Intra-abdominal Lesions.
Iran J Pathol
February 2020
Department of Pathology, N.K.P Salve Institute of Medical Sciences and Research Centre, Nagpur, Maharashtra, India.
Background & Objective: Modified Ultra-fast Papanicolaou (MUFP) stain has been developed from Papanicolaou stain (PAP) with the goal to improve staining quality, minimize staining time for obtaining immediate cytological diagnosis and to check specimen adequacy during Ultrasound guided Fine needle Aspiration Cytology (US guided FNAC). The aim of this research was to study the cytomorphological features of intra-abdominal lesions with help of US guided FNAC and to assess the diagnostic utility of Modified Ultrafast Papanicolaou stain in cytological diagnosis.
Methods: This cross-sectional study enrolled consecutive 100 subjects in N.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!