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OPTiM: Optical projection tomography integrated microscope using open-source hardware and software. | LitMetric

AI Article Synopsis

  • The implementation of an OPT plate for optical projection tomography (OPT) on a commercial wide-field inverted microscope is described, utilizing open-source hardware and software.
  • The OPT plate features a tilt adjustment for alignment and a stepper motor for sample rotation, with three methods detailed for varying magnification needs.
  • The methods include a direct OPT with a limiting aperture, an external optical-relay for high magnifications, and a remote focal scanning approach, all maintaining the microscope's standard light source and functionality while showcasing successful applications on in vivo zebrafish embryos.

Article Abstract

We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope's existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. OPT acquisitions are performed on in vivo zebrafish embryos to demonstrate the implementations' viability.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507440PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180309PLOS

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