Bioactive polyketides and 8,14-seco-ergosterol from fruiting bodies of the ascomycete Daldinia childiae.

Seven previously undescribed polyketides, namely childinins A-G, and one previously undescribed 8,14-seco-ergosterol, namely childinasterone A, were obtained from the fruiting bodies of Daldinia childiae. Their structures and absolute configurations were established via extensive spectroscopic analyses, single-crystal X-ray diffraction, and TDDFT/ECD calculations. Childinins A represents the first example of natural product possessing a previously undescribed 6H-naphtho[2,1-c]chromen-6-one scaffold. The single crystal X-ray diffraction of childinasterone A unambiguously determined the absolute configuration of a 8,14-seco-ergosterol skeleton. Childinins A, B, F and G (MIC 54.9 μg⋅mL) showed anti-bacterial activities. Childinasterone A showed significant anti-NO activity (IC 21.2 μM) and weak activities against SMMC-7721, MCF-7 and SW480 cell lines.