AI Article Synopsis
- The study explored the expression of cytokines IL-17A and IFNγ in gastric biopsies from patients infected with Helicobacter pylori, connecting these findings to disease outcomes such as non-ulcer dyspepsia and gastric ulcers.
- Results indicated that H. pylori infection led to increased expression of IL-17A and IFNγ, particularly in the antrum region, with a stronger presence in patients with gastric ulcers compared to those with non-ulcer dyspepsia.
- The data revealed a correlation between the presence of these cytokines and the level of inflammation in the antrum, highlighting the immune response to H. pylori infection and suggesting a potential role in disease severity.
Article Abstract
Background: Chronic infection with Helicobacter pylori leads to gastritis and in a subpopulation of infected individuals to ulcers and cancer. Bacterial virulence factors and host immune inflammatory responses are risk factors related to disease. CD4 T cells secrete cytokines that promote inflammation and an anti-bacterial response in the gastric mucosa during infection. The aim of the study was to investigate the pattern of expression of CD4 T cell derived cytokines, IL-17A and IFNγ in paired antrum and corpus biopsies and correlate it to H. pylori infection outcome.
Methods: Gene and protein expression of IL-17A and IFNγ was analyzed in gastric biopsies from H. pylori infected subjects with non-ulcer dyspepsia (NUD) or gastric ulcer; and for comparison uninfected individuals.
Results: Upregulation of IL-17A and IFNγ gene expression was seen in corpus and antrum biopsies of H. pylori infected individuals with NUD compared to in uninfected controls. The expression of these cytokines correlated significantly with each other. Immunofluorescence staining revealed increased frequencies of IL-17A and IFNγ cells in antrum biopsies of gastric ulcer patients compared to of H. pylori infected NUD individuals; positive cells were not detected in any of the biopsies of uninfected controls. The frequencies of IFNγ and IL-17A cells correlated positively with inflammation in the antrum, but not the corpus, of H. pylori infected individuals. In the antrum, while there was no significant evidence of correlation between IFNγ and bacterial score, a positive correlation between bacterial score and IL-17A cells was seen.
Conclusions: In H. pylori infected individuals, the frequencies of IFNγ and IL-17A cells were increased in the antrum, particularly in patients with H. pylori induced gastric ulcers. Even though H. pylori colonized both the corpus and antrum regions of the stomach, the cytokine responses and subsequent pathology were mainly detected in the antrum.
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| http://dx.doi.org/10.1016/j.cyto.2017.06.013 | DOI Listing |
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