Sample-to-answer acoustic detection of DNA in complex samples.

Chem Commun (Camb)

Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str, Heraklion, 70013, Greece. and Dept. of Biology, Univ. of Crete, Vassilika Vouton, 71409, Greece.

Published: July 2017

The present study demonstrates the sensitive and label-free acoustic detection of dsDNA amplicons produced from whole Salmonella Thyphimurium cells without employing any DNA extraction and/or purification step, in the presence of the lysed bacterial cells and in a hybridization-free assay. A sample-to-answer assay is also shown during DNA detection directly in milk.

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Source
http://dx.doi.org/10.1039/c6cc10175eDOI Listing

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