MicroRNAs are short RNA molecules that regulate function and stability of a large subset of eukaryotic mRNAs. In the main pathway of microRNA biogenesis, a short "hairpin" is excised from a primary transcript by ribonuclease DROSHA, followed by additional nucleolytic processing by DICER and inclusion of the mature microRNA into the RNA-induced silencing complex. We report that a microRNA-like molecule is encoded by human DROSHA gene within a predicted stem-loop element of the respective transcript. This putative mature microRNA is complementary to DROSHA transcript variant 1 and can attenuate expression of the corresponding protein. The findings suggest a possibility for a negative feedback loop, wherein DROSHA processes its own transcript and produces an inhibitor of its own biosynthesis.
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| http://dx.doi.org/10.1080/15476286.2017.1342934 | DOI Listing |
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Article Synopsis
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- Dysregulation of miRNAs is linked to several Mendelian disorders, such as DICER1 syndrome and neurodevelopmental disorders, with specific examples including hearing loss and skeletal dysplasia.
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