Puumala virus (PUUV), carried by bank voles (Myodes glareolus), is the medically most important hantavirus in Central and Western Europe. In this study, a total of 523 bank voles (408 from Germany, 72 from Slovakia, and 43 from Czech Republic) collected between the years 2007-2012 were analyzed for the presence of hantavirus RNA. Partial PUUV genome segment sequences were obtained from 51 voles. Phylogenetic analyses of all three genome segments showed that the newfound strains cluster with other Central and Western European PUUV strains. The new sequences from Šumava (Bohemian Forest), Czech Republic, are most closely related to the strains from the neighboring Bavarian Forest, a known hantavirus disease outbreak region. Interestingly, the Slovak strains clustered with the sequences from Bohemian and Bavarian Forests only in the M but not S segment analyses. This well-supported topological incongruence suggests a segment reassortment event or, as we analyzed only partial sequences, homologous recombination. Our data highlight the necessity of sequencing all three hantavirus genome segments and of a broader bank vole screening not only in recognized endemic foci but also in regions with no reported human hantavirus disease cases.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11262-017-1484-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Article Synopsis
- Research conducted on 3,400 febrile patients in Peru between 2020-2021 revealed a positive case of Rio Mamore hantavirus, highlighting the endemic presence of this virus in the region.
- Genetic analysis showed a strong similarity to past rodent-associated hantavirus cases from Loreto, indicating ongoing circulation.
- Findings suggest the need for enhanced hantavirus diagnostics and surveillance in Peru and Latin America due to the detection of multiple distinct hantavirus strains.
Phase 1 clinical trial of Hantaan and Puumala virus DNA vaccines delivered by needle-free injection.
NPJ Vaccines
November 2024
Clinical Trials Center, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, USA.
Hantaan virus (HTNV) and Puumala virus (PUUV) are pathogenic zoonoses found in Asia and Europe, respectively. We conducted a randomized Phase 1 clinical trial of individual HTNV and PUUV DNA vaccines targeting the envelope glycoproteins (GnGc), as well as a combined HTNV/PUUV DNA vaccine delivered at varying doses using the PharmaJet Stratis® needle-free injection system (NCT02776761). Cohort 1 and 2 vaccines consisted of 2 mg/vaccination of HTNV or PUUV plasmid, respectively.
View Article and Find Full Text PDFGenetic analysis implicates ERAP1 and HLA as risk factors for severe Puumala virus infection.
Hum Mol Genet
November 2024
Institute for Molecular Medicine Finland, FIMM, HiLIFE, University of Helsinki, Tukholmankatu 8, 00290 Helsinki, Finland.
Article Synopsis
- Puumala virus (PUUV) can lead to serious illnesses like Hemorrhagic Fever with Renal Syndrome, but the genetic factors affecting disease severity in humans are not well understood.
- A genome-wide association study using data from 2227 cases helped identify significant associations at the Human Leukocyte Antigen (HLA) locus and the ERAP1 gene, which are important for immune response.
- The study found a specific genetic variant (rs26653) in the ERAP1 gene and identified associations with several HLA alleles that suggest how these genetic factors may influence susceptibility to severe PUUV infections.
Circulating mucosal-like IgA responses increase with severity of Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome.
Front Immunol
November 2024
Viral Zoonosis Research Unit, Department of Virology, Medicum, University of Helsinki, Helsinki, Finland.
Old World Orthohantaviruses cause hemorrhagic fever with renal syndrome (HFRS) characterized by increased vascular permeability and acute kidney injury (AKI). Despite the systemic nature of the disease, the virus enters humans through inhalation and therefore initially encounters the immunoglobulin class A (IgA) dominated mucosal immune system. Herein, we characterized systemic IgA responses and their potential relationship to the mucosal immune activation by examining blood samples obtained from patients hospitalized due to acute Puumala orthohantavirus infection.
View Article and Find Full Text PDFReplication kinetics of pathogenic Eurasian orthohantaviruses in human mesangial cells.
Virol J
October 2024
Department of Nephrology, University of Heidelberg, Im Neuenheimer Feld 162, D-69120, Heidelberg, Germany.
Background: Eurasian pathogenic orthohantaviruses cause hemorrhagic fever with renal syndrome (HFRS) characterized by acute kidney injury (AKI). The virulence of orthohantaviruses varies enormously and direct infection of different renal cell types contribute to pathogenesis. Glomerular mesangial cells play an essential role in the interplay between kidney cells and proper kidney function.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!