Patulin Induced Oxidative Stress Mediated Apoptotic Damage in Mice, and its Modulation by Green Tea Leaves.

Background: The present study demonstrates the antioxidant and hepatic protective effects of Green tea leaves (GTL).

Methods: The serum level of aspartate aminotransferase and alanine aminotransferase was analyzed. The liver antioxidant enzymes such as SOD, CAT, GPx, GR, GSH, lipid peroxidation and protein carbonyls, ROS content were estimated. The histology of liver tissue was observed and the protein expression of SOD, CAT, Caspase-3 and p53 was investigated by Western blotting.

Results: Effectiveness of GTL extract in preventing patulin induced liver damage showed significant reduction in serum ALT and AST to 19% and 85% respectively, the increase in antioxidant levels and lipid peroxidation products with patulin treatment were also reduced with GTL supplementation. The patulin induced increase in hepatic protein carbonyls was significantly reduced by 141-111% with 100 and 200 mg/kg b.wt GTL and in ROS was significantly reduced by 171-140% with 100 and 200 mg/kg b.wt GTL administration respectively. Also showed protection against hepatic tissue damage and protein expression in mice.

Conclusion: This study showed remarkable antioxidant and hepatic protective effects of GTL.