AI Article Synopsis
- The study looked at how κ-carrageenan polysaccharide influences immune response in RAW 264.7 cells using various assays.
- The results showed that κ-carrageenan not only helps the cells grow but also enhances their ability to engulf pathogens and produce important immune signals like TNF-α.
- Blocking TLR4, a receptor involved in the immune response, reduced the increase in TNF-α, indicating that κ-carrageenan's effects are mediated through TLR4 activation and NF-κB signaling pathways.
Article Abstract
This study investigated the function of κ-carrageenan polysaccharide in immune regulation. The immune response of RAW 264.7 cells treated with κ-carrageenan polysaccharide was explored by MTT assay, general morphological observation, neutral red phagocytosis assay, Griess method, fluorescence method, and enzyme-linked immunosorbent assay. In addition, TLR4 blocking experiment and double-fluorescence immunostaining were performed on cells to demonstrate their immune response mechanism. Results show that κ-carrageenan polysaccharide not only promotes cell proliferation but also activates RAW 264.7 cells, thereby improving the cells' phagocytic capability, NO production, and tumor necrosis factor-α (TNF-α) secretion. In addition, the use of TLR4-specific inhibitors can significantly mediate the increased TNF-α secretion induced by κ-carrageenan polysaccharide. The RAW 264.7 cells treated with κ-carrageenan polysaccharide show upregulated TLR4 expression, and the main subunit of NF-κB (p65) is translocated. These results support the immunomodulatory function of κ-carrageenan polysaccharide in RAW 264.7 cells.
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| http://dx.doi.org/10.1016/j.etap.2017.06.010 | DOI Listing |
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