Betulinic acid is a product of plant secondary metabolism which has shown various bioactivities. Several subfamily genes were recently characterized encoding multifunctional oxidases capable of C-28 oxidation. was identified as betulin C-28 oxidase, capable of modifying betulin. This study aimed to induce the transformation of betulin to betulinic acid by co-expressing enzymes from and from in . The microsome protein extracted from the transgenic yeast successfully catalyzed the transformation of betulin to betulinic acid. We also characterized the optimization of cell fragmentation, protein extraction method, and the conversion conditions. Response surface methodology was implemented, and the optimal yield of betulinic acid reached 18.70%. After optimization, the yield and the conversion rate of betulin were increased by 83.97% and 136.39%, respectively. These results may present insights and strategies for the sustainable production of betulinic acid in multifarious transgenic microbes.
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| http://dx.doi.org/10.3390/molecules22071075 | DOI Listing |
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