AI Article Synopsis
- - The study aimed to see if modifying bone marrow mesenchymal stem cells (BMMSCs) with specific genes could improve their ability to reduce small bowel transplant rejection in Lewis rats.
- - Researchers found that BMMSCs modified with both genes resulted in a median survival time of 53 days for transplant recipients, which was significantly longer compared to modified BMMSCs (39 days), unmodified BMMSCs (26 days), and control groups (16 days).
- - The study concluded that these genetically modified BMMSCs not only reduced rejection rates but also affected cytokine levels, leading to a more favorable immune response after transplantation.
Article Abstract
Aim: To investigate whether bone marrow mesenchymal stem cells (BMMSCs) modified with the and genes can augment the inhibitory effect of BMMSCs on small bowel transplant rejection.
Methods: Lewis rat BMMSCs were cultured . Third-passage BMMSCs were transduced with the genes or the gene alone. The rats were divided into six groups and rats in the experimental group were pretreated with BMMSCs 7 d prior to small bowel transplant. Six time points (instant, 1 d, 3 d, 7 d, 10 d, and 14 d) ( = 6) were chosen for each group. Hematoxylin-eosin staining was used to observe pathologic rejection, while immunohistochemistry and Western blot were used to detect protein expression. Flow cytometry was used to detect T lymphocytes and enzyme linked immunosorbent assay was used to detect cytokines.
Results: The median survival time of BMMSCs from the / modified group (53 d) was significantly longer than that of the modified BMMSCs group (39 d), the BMMSCs group (26 d), and the NS group (control group) (16 d) ( < 0.05). Compared with BMMSCs from the modified BMMSCs, BMMSCs, and NS groups, rejection of the small bowel in the / modified group was significantly reduced, while the weight of transplant recipients was also significantly decreased ( < 0.05). Furthermore, IL-2, IL-6, IL-17, IFN-γ, and TNF-α levels were significantly decreased and the levels of IL-10 and TGF-β were significantly increased ( < 0.05).
Conclusion: BMMSCs modified with the and genes can abrogate the rejection of transplanted small bowel more effectively and significantly increase the survival time of rats that receive a small bowel transplant.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473121 | PMC |
| http://dx.doi.org/10.3748/wjg.v23.i22.4016 | DOI Listing |
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