The purposes of this study were to find the optimum level of supplementing CO for increasing the biomass and hydrocarbon production of B. braunii KMITL 2 (A race) and to determine the effects of CO level on CO fixation and biodiesel properties. The experimental results showed that the alga supplemented with 10% CO produced the highest biomass (1.48±0.02gL) and CO fixation rate (100.43±1.42mgLd) that were 2.7 and 5.3 times higher than the control (0.04% CO). The gravimetric hydrocarbon content (36.82±3.39%) and hydrocarbon titer (0.35±0.03gL) of the alga supplemented with 5% CO were 1.6 and 2.7 times higher than the control. Cultivation of this strain under 5% CO gave the highest hydrocarbon yield and good biodiesel properties with the lowest iodine value and a higher CN value than the ASTM D6751 and EN 14214 fuel standards.
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