The third step of protein splicing is cyclization of Asn coupled to peptide bond cleavage. In two related cyanobacterial inteins, this step is facilitated by Asn or Gln. For a Synechococcus sp. PCC7002 intein, the isolated third step of protein splicing is more efficient with its native Asn than with substitution to Gln. For a Trichodesmium erythraeum intein, its native Gln facilitates the third step as efficiently as with Asn. Despite these differences, the yield of splicing is not affected, suggesting that the third step is influenced by mechanism-linked conformational changes. A conserved catalytic His and the penultimate residue also play roles in promoting side-chain cyclization.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/1873-3468.12730 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Clinical Manifestations.
Alzheimers Dement
December 2024
Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Background: The intricate and heterogeneous phenotypes associated with neuropsychiatric symptoms (NPSs) encumber exploration of their role in the neuropathology and underlying biological mechanisms of Alzheimer's disease (AD) continuum.
Method: An individual-level Regional Radiomics Similarity Network (R2SN) for 487 patients with AD continuum (376 with NPSs vs. 111 without NPSs) were developed to find the R2SN connections associated with NPSs and refine the subtypes of NPS in the AD continuum.
Anisotropic Nanofluidic Ionic Skin for Pressure-Independent Thermosensing.
ACS Nano
January 2025
College of Polymer Science and Engineering, Sichuan University, State Key Laboratory of Polymer Materials Engineering, Chengdu 610065 Sichuan, China.
Ionic skin can mimic human skin to sense both temperature and pressure simultaneously. However, a significant challenge remains in creating precise ionic skins resistant to external stimuli interference when subjected to pressure. In this study, we present an innovative approach to address this challenge by introducing a highly anisotropic nanofluidic ionic skin (ANIS) composed of carboxylated cellulose nanofibril (CNF)-reinforced poly(vinyl alcohol) (PVA) nanofibrillar network achieved through a straightforward one-step hot drawing method.
View Article and Find Full Text PDFInterleaved quartic high gain DC-DC converter.
Sci Rep
January 2025
Power Electronics Research Laboratory (PERL), Faculty of Electrical Engineering, Sahand University of Technology, Tabriz, Iran.
This research paper presents a high-gain DC-DC converter with ultra-step-up voltage gain capability. The proposed converter is synthesized from a two-phase interleaved boost converter (IBC), and its voltage gain is doubled by adopting a voltage lift capacitor. To enhance its voltage gain capability, a floating capacitor-based gain extension cell is adopted subsequently.
View Article and Find Full Text PDFValidity and reliability of the step test to estimate maximal oxygen consumption in pediatric population.
Sci Rep
January 2025
Laboratory of Nutrition and Physical Activity Research (LABINAF), Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, Chile.
Cardiorespiratory fitness is the most important variable related to health and a strong predictor of mortality. However, it is rarely used in clinics due to costs, specialized equipment, space needs, and the requirements of expert staff such as an exercise physiologist, physician, or other health professional. This work aims to validate and test the reliability of a submaximal step test to estimate VOmax of 8-to 16-year-old pediatric populations as a simple and low-cost tool for clinical practice.
View Article and Find Full Text PDFPredicting noncoding RNA and disease associations using multigraph contrastive learning.
Sci Rep
January 2025
College of Information Science Technology, Hainan Normal University, Haikou, 571158, China.
MiRNAs and lncRNAs are two essential noncoding RNAs. Predicting associations between noncoding RNAs and diseases can significantly improve the accuracy of early diagnosis.With the continuous breakthroughs in artificial intelligence, researchers increasingly use deep learning methods to predict associations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!