Dhori and Thogoto viruses have been isolated from ticks in Portugal. In this country favourable ecologic conditions exist for other tick-borne viruses such as Bhanja and Congo-Crimean haemorrhagic fever (C-CHF) viruses. A serological survey for antibodies to these 4 tick-borne viruses in 258 human and 141 goat sera was done using the plaque reduction neutralization (PRN) and indirect fluorescent antibody (IFA) tests. Six human sera were found with antibodies to Dhori (2), Thogoto (2) and C-CHF (2) viruses, while seven goat sera had neutralizing antibodies (NA) to Dhori (5) or Bhanja (2) virus. From these results it seems likely that Bhanja and C-CHF, not yet isolated in Portugal, may exist focally in certain areas of southern Portugal.
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Virology
January 2024
Virology and Cell Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand. Electronic address:
Viruses in the thogotovirus genus of the family Orthomyxoviridae are much less well-understood than influenza viruses despite documented zoonotic transmission and association with human disease. This study therefore developed a cell-cell fusion assay and three pseudotyping tools and used them to assess envelope function and cell tropism. Envelope glycoproteins of Dhori (DHOV), Thogoto (THOV), Bourbon, and Sinu viruses were all revealed to exhibit pH-dependent triggering of membrane fusion.
View Article and Find Full Text PDFJ Gen Virol
August 2023
Institute of Virology, Medical Center and Faculty of Medicine, University of Freiburg, Hermann-Herder-Strasse 11, 79104 Freiburg, Germany.
The recent discovery of Bourbon virus (BRBV) put a new focus on the genus of thogotoviruses as zoonotic, tick-transmitted pathogens within the orthomyxovirus family. Since 2014, BRBV has been linked to several human cases in the Midwest United States with severe acute febrile illness and a history of tick bites. The detection of the virus in the Lone Star tick, , and a high sero-prevalence in wild animals suggest widespread circulation of BRBV.
View Article and Find Full Text PDFJ Virol
March 2022
Institute of Virology, Medical Center-University of Freiburg, Freiburg, Germany.
Thogotoviruses are tick-borne arboviruses that comprise a unique genus within the family. Infections with thogotoviruses primarily cause disease in livestock with occasional reports of human infections suggesting a zoonotic potential. In the past, multiple genetically distinct thogotoviruses were isolated mostly from collected ticks.
View Article and Find Full Text PDFInt J Environ Res Public Health
March 2020
Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, 28801 Alcalá de Henares, Spain.
There is little information on Thogoto virus (THOV) and Dhori virus (DHOV)infection in Spain. A total of 283 serum samples from 150 human subjects (78 males, 72 females) bitten by ticks, as well as samples from 120 sheep (one per animal), were studied by immunofluorescence assay. All human and animal subjects were from the province of Palencia in northern Spain.
View Article and Find Full Text PDFVirol Sin
August 2019
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Baculovirus can transduce a wide range of mammalian cells and is considered a promising gene therapy vector. However, the low transduction efficiency of baculovirus into many mammalian cells limits its practical application. Co-expressing heterologous viral glycoproteins (GPs), such as vesicular stomatitis virus G protein (VSV G), with baculovirus native envelope protein GP64 is one of the feasible strategies for improving virus transduction.
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