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Modification of deglycerolization procedure improves processing and post-thaw quality of cryopreserved sickle trait red cell concentrates.
Cryobiology
June 2024
Innovation and Portfolio Management, Canadian Blood Services, Edmonton, Alberta, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. Electronic address:
Red blood cell (RBC) transfusion is a critical therapy for those with sickle cell disease (SCD). Alloimmunization is frequent for those with SCD and may limit the availability of matched RBC. Cryopreserved RBCs, from family members or donors with a similar RBC antigen profile could provide a viable alternative to avoid further alloimmunization and prevent hemolytic transfusion-related events.
View Article and Find Full Text PDFCompound heterozygosity induces a rare Gerbich-negative phenotype in an immunized blood donor.
Transfusion
February 2024
Laboratory for Immunogenetics / HLA, German Red Cross Blood Service Rhineland-Palatinate and Saarland gGmbH, Bad Kreuznach, Germany.
Background: Antibodies to Gerbich blood group antigens are exceedingly rare and can cause moderate transfusion reactions. Several deletional variants of the GE-gene, that harbors long sequence repeats, enable alloimmunization and formation of naturally occurring antibodies.
Subject And Methods: A female blood donor and soldier of the German Army without history of pregnancy or transfusion showed an antibody reactive with all test cells except for GE:-2-3 RBC.
Recombinantly Expressed Tagged SUrface Protein (RETSUP) assay: a new diagnostic system for the detection of antibodies to platelets.
J Thromb Haemost
April 2024
Institute of Clinical Chemistry and Pathobiochemistry, Klinikum rechts der Isar of the Technische Universität München, Munich, Germany.
Background: Current assays for the detection of (allo)antibodies to platelet antigens are often laborious and widely based on the presence of well-characterized donor platelets.
Objectives: To develop an easy-to-perform, sensitive, and specific test for the detection of antibodies against platelet antigens, in particular, glycoprotein (GP) antigens, called "Recombinantly Expressed Tagged SUrface Protein" (RETSUP) assay, which does not require donor platelets.
Methods: Twin-Strep-tagged GP complexes were recombinantly expressed in human embryonic kidney 293 cells after stable transfection.
A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease.
Eur J Immunol
September 2023
Division of Hematology/Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Article Synopsis
- * This study shows that cryopreservation can effectively preserve lymphoid tissues like human tonsils and lymph nodes for later analysis of LNSCs.
- * The research found that cryopreserved tissue maintained similar cell types and transcriptional profiles to fresh tissue, enabling better investigation of LNSCs in human diseases.
Non-hematopoietic lymph node stromal cells (LNSCs) regulate lymphocyte trafficking, survival, and function for key roles in host defense, autoimmunity, alloimmunity, and lymphoproliferative disorders. However, study of LNSCs in human diseases is complicated by a dependence on viable lymphoid tissues, which are most often excised prior to establishment of a specific diagnosis. Here, we demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease.
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