Int J Oral Maxillofac Implants
Published: June 2017
Purpose: To evaluate the biochemical composition of bone nodules deposited by gingival mesenchymal stem cells (GMSCs) over titanium machined surfaces in vitro.
Materials And Methods: GMSCs were isolated from healthy gingival tissues of patients undergoing crown-lengthening surgical procedures. GMSCs were characterized following the International Society for Cellular Therapy guidelines. After incubation of the GMSCs with titanium discs, osteogenic differentiation was induced for 28 days. Osteogenic lineage was confirmed by means of Alizarin Red S staining. Bone nodule morphology and deposition by GMSCs were characterized by scanning electron microscopy (SEM). An elemental analysis of the bone nodules was done using energy-dispersive x-ray spectroscopy (EDS). The biochemical composition of these nodules was further characterized via Raman spectroscopy, with native alveolar bone used as a control.
Results: GMSCs adhered and proliferated on the titanium discs and exhibited a spindle-shaped fibroblast-like morphology under standard culture conditions. Their phenotype was confirmed by the expression of CD105, CD90, CD73, and CD146, observed using flow cytometry. Deposits of calcium bone nodules were evident in the cultures after staining with Alizarin Red S, but were absent in the controls. Calcium and phosphate, the major components of hydroxyapatite, were present in the bone nodules, as shown by means of the EDS analysis. The results obtained from Raman spectra of these nodules showed the phosphate ions (ν[PO₄³⁻], ~960 cm¹), amide III (δ[NH], ~1,245 cm⁻¹), CH₂ scissors (~1,451 cm⁻¹), amide I (ν[C = O], ~1,667 cm⁻¹), and ν(CH) (2,800-3,100 cm⁻¹) bands were similar to those observed in native bone.
Conclusion: GMSCs can deposit a bone-like mineral highly similar to native bone (HA) over titanium surfaces. Ongoing studies are aimed at determining whether GMSCs can deposit a similar bone matrix/tissue over removed failed dental implants. If HA can be placed over removed failed dental implants, it may be possible to re-osseointegrate dental implants that are failing as a result of peri-implantitis in vivo.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.11607/jomi.5432 | DOI Listing |
Vet Res Commun
January 2025
Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
Protothecosis is a severe, emerging opportunistic infection caused by the saprophytic, achlorophyllous microalgae of the genus Prototheca. Though uncommon, human and animal cases are increasing worldwide, making awareness of this fungal-like pathogen important in both human and veterinary medicine. We report a fatal case of disseminated protothecosis caused by P.
View Article and Find Full Text PDFHua Xi Kou Qiang Yi Xue Za Zhi
February 2025
Dept. of Cariology and Endodontics, Binzhou Medical University Hospital, Binzhou 256600, China.
Objectives: The mechanism of the odontogenic differentiation of apical papillary cells (APCs) stimulated by bioactive glass 45S5 is still unclear. This study aims to investigate the effect of autophagy on the odontogenic differentiation of APCs stimulated by bioactive glass 45S5.
Methods: APCs were isolated and cultured , and the cell origin was identified by flow cytometry.
Front Oncol
January 2025
Department of Thyroid Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.
Background: It is uncommon to come across instances of aplastic anemia in individuals suffering from papillary thyroid carcinoma complicated by Hashimoto's thyroiditis. Here, a unique case is presented.
Case Presentation: A 23-year-old male was admitted to the hospital for "a lump in his right neck".
Biomedica
December 2024
Laboratorio de Inmunodeficiencias, Instituto Nacional de Pediatría, Ciudad de México, México.
Chronic granulomatous disease is the inborn error of immunity with the highest frequency of invasive aspergillosis. In this context, invasive aspergillosis is frequent in adolescence, with rare cases before one year of age. We present a case of chronic granulomatous disease and invasive aspergillosis in a four-month-old infant.
View Article and Find Full Text PDFJ Oral Biosci
January 2025
Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan. Electronic address:
Objectives: Low-level laser therapy (LLLT) using an erbium-doped yttrium aluminum garnet (Er:YAG) laser provides a non-invasive approach applicable to various dental treatments. Here, we investigated the effects of Er:YAG laser irradiation on human dental pulp stem cells (hDPSCs) in an in vitro experiment.
Methods: The hDPSCs were categorized into four groups: laser-irradiated with activators (VLT: activated vitamin D, bone morphogenetic protein receptor inhibitor, and transforming growth factor-beta (TGF-β)) (LLLT(+)VLT), laser-irradiated without activators (LLLT(+)-only), non-irradiated with activators (LLLT(-)VLT), and non-irradiated without activators (control).
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.