A rapid, simple, and novel method for folate receptor (FR) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FR antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FR was added to the probe for the specific interaction between antibody and antigen, resulting in the enhancement of resonance Rayleigh scattering (RRS) intensity. There is a linear relationship between the change of RRS intensity (Δ) and the concentration of FR, with the detecting range of 0.50-37.50 ng·mL and the limit of determination of 0.05 ng·mL. The determination of FR in serum samples was realized with the advantages of high selectivity, high sensitivity, and easy operation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5463099 | PMC |
| http://dx.doi.org/10.1155/2017/1670812 | DOI Listing |
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