This corrects the article DOI: 10.1038/nplants.2017.18.

Download full-text PDF

Source
http://dx.doi.org/10.1038/nplants.2017.103DOI Listing

Publication Analysis

Top Keywords

crispr-cpf1 system
4
system efficient
4
efficient genome
4
genome editing
4
editing transcriptional
4
transcriptional repression
4
repression plants
4
plants corrects
4
corrects article
4
article doi
4

Similar Publications

Optogenetic control of Corynebacterium glutamicum gene expression.

Nucleic Acids Res

December 2024

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.

Article Synopsis
  • Corynebacterium glutamicum is an important microorganism used in the production of amino acids and valuable chemicals, highlighting the need for better tools to regulate its gene expression for improved efficiency.
  • This study introduces 'LightOnC.glu', a cutting-edge light-controlled gene expression system utilizing RNA-binding proteins and CRISPR/Cpf1 tools to precisely manage gene activity in C. glutamicum.
  • The research successfully enables the production of chitin and chondroitin sulphate oligosaccharides, achieving a record titer of 6.2 g/L, showcasing the potential of programmable light-responsive genetic circuits for enhancing production processes.
View Article and Find Full Text PDF

Treatment of (Mab) infections is very challenging due to its intrinsic resistance to most available drugs. Therefore, it is crucial to discover novel anti-Mab drugs. In this study, we explored an intrinsic resistance mechanism through which Mab resists echinomycin (ECH).

View Article and Find Full Text PDF

Establishment of the CRISPR-Cpf1 gene editing system in and multiplexed gene knockout.

Synth Syst Biotechnol

August 2024

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China.

is a significant industrial microorganism. Traditional gene editing techniques relying on homologous recombination often exhibit low efficiency due to their reliance on resistance genes. Additionally, the established CRISPR gene editing technology, utilizing Cas9 endonuclease, faces challenges in achieving simultaneous knockout of multiple genes.

View Article and Find Full Text PDF

Cyanobacteria have great potential in CO-based bio-manufacturing and synthetic biological studies. The filamentous cyanobacterium, sp. strain BL0902, is comparable to () in commercial-scale cultivation while proving to be more genetically tractable.

View Article and Find Full Text PDF

CRISPR-Cpf1 system and its applications in animal genome editing.

Mol Genet Genomics

August 2024

Stem Cells and Biotherapy Engineering Research Center of Henan, National Joint Engineering Laboratory of Stem Cells and Biotherapy, School of Life Science and Technology, Xinxiang Medical University, Number 601, Jinsui Road, Xinxiang, 453003, Henan, China.

The clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein (Cas) system is a gene editing technology guided by RNA endonuclease. The CRISPR-Cas12a (also known as CRISPR-Cpf1) system is extensively utilized in genome editing research due to its accuracy and high efficiency. In this paper, we primarily focus on the application of CRISPR-Cpf1 technology in the construction of disease models and gene therapy.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!