Highly efficient regeneration system has been developed for Burkill, an important herb used as substitute for . Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.4 ± 0.8) were obtained on MS media containing TDZ (3.0 mg l) in combination with IBA (0.3 mg l). Maximum callus induction (95 ± 4.8%) and callus growth (1.7 ± 0.4 gm) was achieved on MS medium with 2, 4-D (3.0 mg l). Cell suspension cultures were established and studied for their growth kinetics. Shoots were rooted best (22.1 ± 2.5) in 1/2 MS medium with IAA (3.0 mg l). The genetic uniformity of the micropropagated clones was assessed using RAPD markers. Out of 405 bands, 400 (98.76%) were monomorphic and rest 5 (1.24%) were polymorphic. High multiplication frequency and low risk of genetic instability ensures the efficacy of this protocol.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466263 | PMC |
| http://dx.doi.org/10.1016/j.btre.2015.03.003 | DOI Listing |
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