A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA).
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http://dx.doi.org/10.1016/j.btre.2014.06.008 | DOI Listing |
Heliyon
December 2024
Chongqing Jiangbei District Disease Control Center, Chongqing, 400020, China.
Currently, the utilization value of plantain straw is low. To increase its value, plantain straw was utilized in this study to produce xylooligosaccharides (XOS). XOS were obtained from plantain straw through xylanase hydrolysis.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Anhui Provincial Engineering Center for High Performance Biobased Nylons, School of Materials and Chemistry, Anhui Agricultural University, Hefei 230036, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address:
Developing a mild and efficient pretreatment technique to fully utilize lignocellulosic biomass remains a challenge. In this work, a biphasic system with 2-phenoxyethanol (EPH) organic solvent and phosphotungstic acid (PTA) aqueous solution was employed to pretreat corn stover. The prominent synergistic effect between EPH and PTA was revealed to play a key role in the fractionation of corn stover.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China. Electronic address:
As an "upstream" process in biorefinery, biomass dismantling can dismantle the natural stable structure of lignocellulosic biomass and separate its three major components. To increase the value of the entire biomass by fully utilizing the three main components (cellulose, lignin, and hemicellulose), this study proposes a two-step decomposition system combining formic acid (FA) pretreatment and ethylene glycol-NaOH (EGA) dismantling, aiming to effectively convert sugarcane bagasse into xylose, fermentable glucose, and high-value lignin. In the first step, FA pretreatment removed 79.
View Article and Find Full Text PDFBiochemistry
January 2025
Department of Biochemistry & Molecular Biology, University of Georgia, Athens, Georgia 30602, United States.
Human UDP-glucose dehydrogenase (hUGDH) catalyzes the oxidation of UDP-glucose into UDP-glucuronic acid, an essential substrate in the Phase II metabolism of drugs. hUGDH is a hexamer that exists in an equilibrium between an active (E) state and an inactive (E) state, with the latter being stabilized by the binding of the allosteric inhibitor UDP-xylose (UDP-Xyl). The allosteric transition between E and E is slow and can be observed as a lag in progress curves.
View Article and Find Full Text PDFMicrob Cell Fact
December 2024
College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
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