AI Article Synopsis
- * This study presents structural snapshots of the glucosyl-3-phosphoglycerate synthase (GpgS) enzyme, detailing how it positions the substrates for the glycosyl transfer reaction and the subsequent release of products.
- * Additional calculations reveal how conformational changes in both the substrates and the enzyme influence the overall reaction process, enhancing our understanding of GT enzymes at the molecular level.
Article Abstract
Glycosyltransferases (GTs) play a central role in nature. They catalyze the transfer of a sugar moiety to a broad range of acceptor substrates. GTs are highly selective enzymes, allowing the recognition of subtle structural differences in the sequences and stereochemistry of their sugar and acceptor substrates. We report here a series of structural snapshots of the reaction center of the retaining glucosyl-3-phosphoglycerate synthase (GpgS). During this sequence of events, we visualize how the enzyme guides the substrates into the reaction center where the glycosyl transfer reaction takes place, and unveil the mechanism of product release, involving multiple conformational changes not only in the substrates/products but also in the enzyme. The structural data are further complemented by metadynamics free-energy calculations, revealing how the equilibrium of loop conformations is modulated along these itineraries. The information reported here represent an important contribution for the understanding of GT enzymes at the molecular level.
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| http://dx.doi.org/10.1016/j.str.2017.05.009 | DOI Listing |
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