AI Article Synopsis
- Researchers studied Tetraselmis sp. M8 to understand how lipid pathways contribute to rapid triacylglyceride buildup after nitrogen exhaustion.
- RNA-Seq and real-time PCR showed a significant change in gene expression related to fatty acid/triacylglyceride production and breakdown during different growth phases.
- The findings suggest that increased lipid accumulation occurs due to reduced breakdown of lipids and enhanced production during the stationary phase, independent of the DGAT gene, which is typically important for lipid storage.
Article Abstract
To map out key lipid-related pathways that lead to rapid triacylglyceride accumulation in oleaginous microalgae, RNA-Seq was performed with Tetraselmis sp. M8 at 24h after exhaustion of exogenous nitrogen to reveal molecular changes during early stationary phase. Further gene expression profiling by quantitative real-time PCR at 16-72h revealed a distinct shift in expression of the fatty acid/triacylglyceride biosynthesis and β-oxidation pathways, when cells transitioned from log-phase into early-stationary and stationary phase. Metabolic reconstruction modeling combined with real-time PCR and RNA-Seq gene expression data indicates that the increased lipid accumulation is a result of a decrease in lipid catabolism during the early-stationary phase combined with increased metabolic fluxes in lipid biosynthesis during the stationary phase. During these two stages, Tetraselmis shifts from reduced lipid consumption to active lipid production. This process appears to be independent from DGAT expression, a key gene for lipid accumulation in microalgae.
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| http://dx.doi.org/10.1016/j.biortech.2017.06.003 | DOI Listing |
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