AI Article Synopsis
- The study focuses on generating glutathione directly from mannan-based materials using genetically modified yeast (Saccharomyces cerevisiae).
- The researchers engineered the yeast to express specific enzymes that break down mannan, allowing it to efficiently convert mannose polysaccharides into glutathione.
- They found that using locust bean gum, a cheap source of mannan, significantly boosted glutathione production, highlighting a potential eco-friendly method for bioproduction from such resources.
Article Abstract
This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, β-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a highly dense and inexpensive mannan-based bioresource, as sole carbon source. Glutathione productivity from LBG was enhanced by engineering the glutathione metabolism of mannan-hydrolyzing S. cerevisiae. Expression of extracellular mannanase/mannosidase protein combined with intracellular metabolic engineering is potentially applicable to the efficient, environmentally friendly bioproduction of targeted products from mannan-based bioresources.
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| http://dx.doi.org/10.1016/j.biortech.2017.05.190 | DOI Listing |
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