A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria.

Antibacterial compounds are widely used in the treatment of human and animal diseases. The overuse of antibiotics has led to a rapid rise in the prevalence of drug-resistant bacteria, making the development of new antibacterial compounds essential. This study focused on developing a fast and easy method for identifying marine bacteria that produce antibiotic compounds. Eight randomly selected marine target bacterial species (, and ) were tested for production of antibacterial compounds against four strains of test bacteria (, and ). Colony picking was used as the primary screening method. Clear zones were observed around colonies of , and tested against , and . The efficiency of colony scraping and broth culture methods for antimicrobial compound extraction was also compared using a disk diffusion assay. , and showed antagonistic activity against , and , respectively, only in the colony scraping method. Our results show that colony picking and colony scraping are effective, quick, and easy methods of screening for antibacterial compound-producing bacteria.