AI Article Synopsis
- A new fluorescence-based assay has been created to measure alkaline phosphatase (ALP) activity, using polyethyleneimine-capped copper nanoclusters (PEI-capped CuNCs) and manganese oxide (MnO) nanosheets.
- When in solution, the positively charged CuNCs stick to the negatively charged MnO, which reduces the fluorescence from the CuNCs due to energy transfer between them.
- When ALP is present, it converts a specific compound into L-ascorbic acid, which then reduces MnO, releasing the CuNCs and restoring their fluorescence; this method is simple, cost-effective, sensitive, selective, and has potential for various biological applications.
Article Abstract
A fluorescence turn-on assay for alkaline phosphatase (ALP) activity is developed through the controlled release of polyethyleneimine-capped copper nanoclusters (PEI-capped CuNCs) from the MnO nanosheets. In an aqueous solution, the positively charged PEI-capped CuNCs could be adsorbed onto the surface of the negatively charged MnO nanosheets. Such adsorption through favorable electrostatic interactions could efficiently quench the nanocluster fluorescence emission via resonance energy transfer from the PEI-capped CuNCs to the MnO nanosheets. 2-Phospho-L-ascorbic acid (AAP) could be hydrolyzed to L-ascorbic acid (AA) in the presence of ALP. AA could reduce MnO into Mn and trigger the disintegration of the MnO nanosheets. As a result, the CuNCs were released and the quenched fluorescence was recovered efficiently. The detection strategy is simple, inexpensive, sensitive, selective, with low toxicity, and has better biocompatibility. The newly fabricated biosensor for ALP activity will potentially make it a robust candidate for numerous biological and biomedical applications.
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| http://dx.doi.org/10.1007/s00216-017-0420-9 | DOI Listing |
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