We have shown that the maximum achievable resolution of an in-line lensless holographic microscope is limited by aliasing and, for collimated illumination, cannot exceed the camera pixel size. This limit can be achieved only when the optimal conditions on the spatial and temporal coherence state of the illumination are satisfied. The expressions defining the configuration, delivering maximum resolution with given spatial and temporal coherence of the illumination, are obtained. The validity of these conditions is confirmed experimentally.

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.42.002271DOI Listing

Publication Analysis

Top Keywords

lensless holographic
8
holographic microscope
8
spatial temporal
8
temporal coherence
8
aliasing coherence
4
coherence resolution
4
resolution lensless
4
microscope maximum
4
maximum achievable
4
achievable resolution
4

Similar Publications

A fair comparison of multiple live cell cultures requires examining them under identical environmental conditions, which can only be done accurately if all cells are prepared simultaneously and studied at the same time and place. This contribution introduces a multiplexed lensless digital holographic microscopy system (MLS), enabling synchronous, label-free, quantitative observation of multiple live cell cultures with single-cell precision. The innovation of this setup lies in its ability to robustly compare the behaviour, i.

View Article and Find Full Text PDF

A lensless compact arrangement based on digital in-line holography under Gabor's regime is proposed as a novel contactless method to assess the profile of multifocal intraocular lenses (MIOLs) which are conformed by several diffractive rings. Diffractive MIOLs are a widely adopted ophthalmologic option for the correction of presbyopia in patients undergoing cataract surgery. The MIOL optical design might introduce non-negligible optical performance differences between lenses as well as the introduction of undesirable photic phenomena (such as halos and glare) affecting the vision of users.

View Article and Find Full Text PDF

Lensless microscopy is an imaging technique that allows high-resolution imaging over a large field of view with a cost-effective design. Conventional lensless microscopy often utilizes multi-height phase retrieval and pixel-super-resolution algorithms to reconstruct high-resolution images, requiring mechanical stages for three-dimensional relative movements between a light source, camera, and sample. However, the excessive use of stages inevitably increases the bulkiness of the system and extends the image acquisition time.

View Article and Find Full Text PDF

Optimization-based phase retrieval method for digital lensless holographic microscopy in the double-plane recording configuration is proposed. In our method the phase retrieval is framed as an optimization problem that can be efficiently and rigorously tackled with gradient decent tools. This is done with the conjugate gradient method that possesses excellent theoretical features such as global and fast convergence (compared to steepest descent) and relatively low computational cost (compared to second order optimizers).

View Article and Find Full Text PDF

In this Letter, we propose a new, to the best of our knowledge, lensless on-chip holographic microscopy platform, which can acquire sub-pixel-shifting holograms through centimeter (cm)-level lateral translations. An LED light source is used to illuminate the sample, and two orthogonally tilted step-structure glass plates are inserted into the optical path. By merely displacing the glass plates under cm-level precision, a series of holograms with sub-pixel displacements can be obtained.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!