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State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao, China.

This review focused on mass spectrometry imaging (MSI), a powerful tool in food analysis, covering its ion source schemes and procedures and their applications in food quality, safety, and nutrition to provide detailed insights into these aspects. The review presented a detailed introduction to both commonly used and emerging ionization sources, including nanoparticle laser desorption/ionization (NPs-LDI), air flow-assisted ionization (AFAI), desorption ionization with through-hole alumina membrane (DIUTHAME), plasma-assisted laser desorption ionization (PALDI), and low-temperature plasma (LTP). In the MSI process, particular emphasis was placed on quantitative MSI (QMSI) and super-resolution algorithms.

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3D Cryo-Correlative Methods to Study Virus Structure and Dynamics Within Cells.

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Department of Macromolecular Structure, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain.

Understanding the dynamic processes involving virus structural components within host cells is crucial for comprehending viral infection, as viruses rely entirely on host cells for replication. Viral infection involves various intracellular stages, including cell entry, genome uncoating, replication, transcription and translation, assembly of new virus particles in a complex morphogenetic process, and the release of new virions from the host cell. These events are dynamic and scarce and can be obscured by other cellular processes, necessitating novel approaches for their in situ characterization.

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We report a method for generating uniform, artifact-free total internal reflection fluorescence (TIRF) excitation via a photonic lantern. Our tapered waveguide, consisting of a multimode input and nine few-mode outputs, enables single-shot TIRF illumination from nine azimuthal directions simultaneously without the introduction of nonstationary devices. Utilizing the photonic lantern for multi-beam excitation provides a low-loss mechanism that supports a wide range of light sources, including high-coherence lasers and various wavelengths in the visible spectrum.

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Article Synopsis
  • Terahertz (THz) waves have significant potential applications in military, industrial, and biomedical sectors, but challenges such as spatial limits and image clarity hinder their effectiveness.
  • Thz time-domain spectroscopy (THz-TDS) imaging systems analyze samples via spectral signals, yet face difficulties with atmospheric absorption and noise.
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  • MRI is essential for diagnosing and monitoring multiple sclerosis (MS), but standard scans often have limited resolution due to thick slices, which affects automated analysis.
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