Assessment of nanoparticles' safety: corrected absorbance-based toxicity test.

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Powder Technology Laboratory, Institute of Materials, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Published: June 2017

AI Article Synopsis

  • Developed a method to adjust absorbance-based toxicity tests for the impact of nanoparticle doses.
  • Used the MTS test as a model for these adjustments.
  • The corrected results from the MTS test aligned with the findings from the fluorescence-based PrestoBlue® test.

Article Abstract

We developed a method to correct absorbance-based toxicity tests to remove the influence of the deposited dose of nanoparticles. As a model of absorbance-based toxicity tests, we chose the frequently used MTS test. The corrected cell viabilities obtained with the MTS test matched the results of the fluorescence-based PrestoBlue® test.

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Source
http://dx.doi.org/10.1039/c7an00382jDOI Listing

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