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Targeted Assay Engineering Enhances Bile Salt Hydrolase Activity in Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012.
Curr Microbiol
January 2025
Department of Food Engineering and Technology, Institute of Chemical Technology, Matunga, Mumbai, Maharashtra, India.
The recent emergence of bile salt hydrolase (BSH) enzyme as a therapeutic target reflects its unbound potential in mitigating hypercholesterolemia, obesity, and gastrointestinal issues. However, to bolster its industrial application, optimization of BSH assay lays the cornerstone for enhancing sensitivity, specificity, and reproducibility. The current study delved into optimizing the BSH assay parameters utilizing response surface methodology (RSM) and one-factor-at-a-time (OFAT) method for two novel, natural BSH producers, Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012.
View Article and Find Full Text PDFGenetic Basis and Identification of Candidate Genes for Alkalinity Tolerance Trait in Spotted Sea Bass (Lateolabrax maculatus) by Genome-Wide Association Study (GWAS).
Mar Biotechnol (NY)
January 2025
Key Laboratory of Mariculture, Ministry of Education (KLMME), Ocean University of China, Qingdao, 266003, China.
Given the challenges of overcrowded coastal aquaculture spaces and insufficient production, utilizing saline-alkaline water areas represents a vital strategy to alleviate these bottlenecks. Spotted sea bass (Lateolabrax maculatus), with its formidable osmoregulatory capabilities, is an ideal candidate to develop a saline-alkaline tolerant strain. In our study, genotypic and phenotypic data from 287 L.
View Article and Find Full Text PDFTetramethylguanidine-Modified Graphene Oxide as a Gel Polymer Electrolyte Additive for Improving the Performance of Flexible Zinc-Air Batteries.
Small
January 2025
School of Mechanical Engineering, Guangxi University, Nanning, 530004, P. R. China.
Flexible zinc-air batteries (FZABs) present a promising solution for the next generation of power sources in wearable electronics, owing to their high energy density, cost-effectiveness, and safety. However, solid-state electrolytes for FZABs continue to face challenges related to rapid water loss and low ionic conductivity. In this study, a hydrophilic and stable tetramethylguanidine-modified graphene oxide as an additive, which is incorporated into sodium polyacrylate to develop a high-performance gel polymer electrolyte (GPE), is designed.
View Article and Find Full Text PDFEffect of different protocols in preventing demineralization in irradiated human enamel, in vitro study.
BMC Oral Health
January 2025
Department of Operative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Background: This in vitro study evaluated the efficacy of professional and home-use fluoride regimens for protecting irradiated enamel, undergoing pH cycling resembling xerostomia.
Methods: Sixty human premolar teeth were irradiated with a total dose of 70 Gy and subsequently sectioned into 3 × 3 cm enamel slabs. These slabs were randomly distributed into five groups (n = 12 per group): professional-use groups received fluoride varnish either weekly (FV1) or biweekly (FV2); home-use groups applied 5000 ppm (FT5) or 1450 ppm (FT) fluoride toothpaste; and a control group (control) received no treatment.
Biodegradation of azo dyes by Aspergillus flavus and its bioremediation potential using seed germination efficiency.
BMC Microbiol
January 2025
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Cairo, 11341, Egypt.
The worldwide textile industry extensively uses azo dyes, which pose serious health and environmental risks. Effective cleanup is necessary but challenging. Developing bioremediation methods for textile effluents will improve color removal efficiency.
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