Hypothesis: Predicting formation mode of double emulsion drops in microfluidic emulsification is crucial for controlling the drop size and morphology.
Experiments And Modelling: A three-phase Volume of Fluid-Continuum Surface Force (VOF-CSF) model was developed, validated with analytical solutions, and used to investigate drop formation in different regimes. Experimental investigations were done using a glue-free demountable glass capillary device with a true axisymmetric geometry, capable of readjusting the distance between the two inner capillaries during operation.
Findings: A non-dimensional parameter (ζ) for prediction of double emulsion formation mode as a function of the capillary numbers of all fluids and device geometry was developed and its critical values were determined using simulation and experimental data. At logζ>5.7, drops were formed in dripping mode; the widening jetting occurred at 5
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http://dx.doi.org/10.1016/j.jcis.2017.05.115 DOI Listing Publication Analysis
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Microbiology (Reading)
January 2025
Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, USA.
Sphingoid bases, including sphingosine, are important components of the antimicrobial barrier at epithelial surfaces where they can cause growth inhibition and killing of susceptible bacteria. is a common opportunistic pathogen that is less susceptible to sphingosine than many Gram-negative bacteria. Here, we determined that the deletion of the operon reduced growth in the presence of sphingosine.
View Article and Find Full Text PDFBackground: Phosphorylated tau (p-tau) is a specific blood biomarker for Alzheimer disease (AD) pathology, with p-tau217 having the greatest utility. Increased and simplified access to blood biomarkers is crucial for early diagnosis, proper patient management and prompt initiation of disease-modifying treatments. The DROP-AD project investigates the capability of finger-prick collection to accurately measure p-tau217, neurofilament light (NfL), and glial fibrillary acidic protein (GFAP).
View Article and Find Full Text PDFBackground: E2814 is a humanized monoclonal antibody that recognizes the microtubule-binding region (MTBR) of tau, a region of the protein essential for filament formation and propagation in neurodegenerative diseases. Epitope mapping showed that E2814 binds to a specific sequence motif HVPGG in the MTBR. To elucidate the atomic interactions of E2814-tau binding, we performed X-ray crystallography studies with E2814 and various tau peptides containing the HVPGG motif.
View Article and Find Full Text PDFBackground: Amylin is a systemic hormone that is co-secreted with insulin from pancreatic β-cells. Amylin co-aggregates with brain parenchymal and vascular β-amyloid in persons with Alzheimer's dementia. The present pilot study sought to assess the safety and side effects during and after the treatment period of passive amylin immunotherapy in the APP/PS1 mouse model of Alzheimer's disease.
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Burke Neurological Institute, Weill Cornell Medicine, White Plains, NY, USA.
Background: Benfotiamine, a prodrug of thiamine, raises blood levels by 50-100 times to achieve pharmacologic effects. It provides a novel therapeutic direction addressing a well-characterized brain tissue thiamine deficiency and related changes in glucose metabolism in AD. BenfoTeam is a seamless phase 2A-2B "proof of concept" (POC), double-blind, placebo-controlled RCT investigating tolerability, safety, and efficacy of benfotiamine, as a first-in-class small molecule treatment for early AD.
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