[Microbial Community Structure on the Root Surface of Patients with Periodontitis.].

Objectives: To study the microbial community structure on the root surface of patients with periodontitis.

Methods: Bacterial plaque and tissues from the root neck (RN group),root middle (RM group) and root tine (RT group) of six teeth with mobility 3 in one patient with periodontitis were sampled.The V3V4 region of 16S rRNA was sequenced on the Illumina MiSeq platform.The microbial community structure was analyzed by Mothur,Qiime and SPSS software.

Results: The principal component analysis (PCoA) results indicated that the RM samples had a similar microbial community structure as that of the RT samples,which was significant different from that of the RN samples.Thirteen phyla were detected in the three groups of samples,which included 7 dominant phyla.29 dominant genera were detected in 184 genera.The abundance of _[G-6] and _[XI][G-4] had a positive correlation with the depth of the collection site of samples (<0.05),while the abundance of and had a negative correlation with the depth of the collection site of samples (<0.05).

Conclusions: There is region-specificity of microbial community structure on the root surface of patients with periodontitis.