AI Article Synopsis
- The study aimed to evaluate how the sphingosine-1-phosphate receptor 2 (S1PR2) influences vascular permeability in mice with acute lung injury.
- Using models that involved intratracheal administration of lipopolysaccharide (LPS), researchers assessed the effects of S1PR2 through various measurements of fluid leakage in the lungs.
- Results showed that mice lacking S1PR2 experienced greater vascular leakage and edema following LPS treatment, indicating that S1PR2 plays a protective role in maintaining the integrity of endothelial cell barriers.
Article Abstract
Objectives: To determine the effect of sphingosine-1-phosphate receptor 2 (S1PR2) on vascular permeability in mice.
Methods: Acute lung injury models of mice were constructed with intra-tracheal administration of lipopolysaccharide (LPS) and compared with the controls with intra-tracheal administration of saline. The effect of S1PR2 on vascular permeability was observed by detecting leakage of Evans blue into lung tissues, pulmonary vascular leakage of fluorescein isothiocyanate (FITC)-dextran, and the wet/dry mass ratio of lungs. The effect of vascular endothelial growth factor (VEGF) on vascular endothelial permeability was detected by Miles analysis.
Results: LPS injections induced significant Evans blue leakage, FITC-dextran pulmonary vascular leakage and pulmonary edema, which appeared to be more serious in S1PR2-deleted mice compared with those in wild-type mice. LPS enhanced Evans blue leakage associated with VEGF in a dose-dependent way in both S1PR2-deleted mice and wild type mice. But the vascular permeability response in subcutaneous tissues induced by VEGF was higher in S1PR2-deleted mice than that in wild-type mice.
Conclusions: S1PR2 is involved in endothelial cell barrier protections, which inhibits vascular permeability.
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