AI Article Synopsis
- * The RcsA gene was isolated using PCR techniques, revealing that its protein structure contains conserved domains similar to those found in other bacterial activators.
- * Mutants lacking RcsA showed significantly reduced EPS production and lost their ability to cause disease in mushrooms, highlighting RcsA's critical role as a virulence factor.
Article Abstract
RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the . The gene of the soft rot pathogen sp. strain PPE7 in was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins. The inactivation of by insertional mutagenesis created mutants that had decreased production of EPS compared to the wild-type strain and abolished the virulence of sp. strain PPE7 in . The sp. strain PPE7 gene was shown to strongly affect the formation of the disease symptoms of a mushroom pathogen and to act as the virulence factor to cause soft rot disease in .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461046 | PMC |
| http://dx.doi.org/10.5423/PPJ.OA.08.2016.0177 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!