Objective: The present study aims to evaluate antioxidants and protective role of Linn. against oxidative stress-induced DNA and cell membrane damage.
Materials And Methods: The total and profiles of flavonoids were identified and quantified through reversed-phase high-performance liquid chromatography. antioxidant activity was determined using standard antioxidant assays. The protective role of extracts against oxidative stress-induced DNA and cell membrane damage was examined by electrophoretic and scanning electron microscopic studies, respectively.
Results: The total flavonoid content of CtEA was 106.8 ± 2.8 mg/g d.w.QE, CtME was 72.4 ± 1.12 mg/g d.w.QE, and CtWE was 30.4 ± 0.8 mg/g d.w.QE. The concentration of flavonoids present in CtEA in decreasing order: quercetin >kaempferol >epicatechin; in CtME: quercetin >rutin >kaempferol; whereas, in CtWE: quercetin >rutin >kaempferol. The CtEA inhibited free radical-induced red blood cell hemolysis and cell membrane morphology better than CtME as confirmed by a scanning electron micrograph. CtEA also showed better protection than CtME and CtWE against free radical-induced DNA damage as confirmed by electrophoresis.
Conclusion: contains flavonoids and inhibits oxidative stress and can be used for many health benefits and pharmacotherapy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450468 | PMC |
http://dx.doi.org/10.4103/0975-7406.206215 | DOI Listing |
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