The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mimet.2017.06.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris.
J Vis Exp
January 2025
Department of Molecular Cellular and Developmental Biology, University of California, Santa Barbara; Neuroscience Research Institute, University of California, Santa Barbara.
The tardigrade Hypsibius exemplaris is an emerging model organism renowned for its ability to survive environmental extremes. To explore the molecular mechanisms and genetic basis of such extremotolerance, many studies rely on RNA-sequencing (RNA-seq), which can be performed on populations ranging from large cohorts to individual animals. Reverse transcription polymerase chain reaction (RT-PCR) and RNA interference (RNAi) are subsequently used to confirm RNA-seq findings and assess the genetic requirements for candidate genes, respectively.
View Article and Find Full Text PDFChest tube provocative clamping in patients having moderate or intense air leaks after lung resection to accelerate recovery.
J Thorac Dis
December 2024
Guangdong Lung Cancer Institute, Guangdong Provincial Key Laboratory of Translational Medicine in Lung Cancer, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Southern Medical University, Guangzhou, China.
Background: Postoperative air leak is the most common complication after pulmonary resection. "Provocative clamping" was first described in 1992 in the context of guiding chest tube removal despite persistent air leak. However, early provocative clamping after pulmonary resection has not been evaluated.
View Article and Find Full Text PDFThoracic duct identification using three-dimensional thoracoscope versus indocyanine green fluorescence during minimally invasive esophagectomy: a retrospective cohort study.
J Thorac Dis
December 2024
Department of Thoracic Surgery, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, China.
Background: Chylothorax following esophagectomy is a frustrating complication with considerable morbidity. In addition, recognizing the morphological patterns of the thoracic duct (TD) holds great significance. This study was aimed at explore the safety and efficacy of three-dimensional (3D) thoracoscope in comparison with indocyanine green (ICG) fluorescence to identify TD during minimally invasive esophagectomy (MIE) for esophageal cancer.
View Article and Find Full Text PDFCoupling Study of Fracture Propagation-Filtration-Seepage during Hydraulic Fracturing Assisted Oil Displacement in Offshore Low-Permeability Reservoirs.
ACS Omega
January 2025
Laboratory of Enhanced Oil Recovery of Education Ministry, Northeast Petroleum University, Daqing, Heilongjiang 163318, China.
Offshore low-permeability reservoirs are mainly composed of complex fault-block structures with poor physical properties, which makes establishing an effective displacement relationship particularly challenging. Hydraulic fracturing assisted oil displacement (HFAD) can effectively increase the oil production of a single well by creating fractures to replenish the producing energy. In this study, the Khristianovich-Geertsma-de Klerk (KGD) model is used to calculate the propagation of vertical fractures, and the flow tube method is used to calculate the two-phase oil-water flow in filtration and seepage.
View Article and Find Full Text PDFA combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4.
Sci Rep
January 2025
Department of Plant Pathology, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
The soilborne pathogen Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is currently devastating banana production worldwide.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!