Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity. | LitMetric
Dept. of Psychology & Neuroscience Program, University of Michigan, Ann Arbor, MI, USA. Electronic address:
Published: September 2017
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Article Abstract
The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. To advance research on the impact of CHT capacity in humans, we established the presence of the neuronal CHT protein in human T lymphocytes. Next, we demonstrated CHT-mediated choline transport in human T cells. To address the validity of T cell-based choline uptake as a proxy for brain CHT capacity, we isolated T cells from the spleen, and synaptosomes from cortex and striatum, of wild type and CHT-overexpressing mice (CHT-OXP). Choline uptake capacity in T cells from CHT-OXP mice was two-fold higher than in wild type mice, mirroring the impact of CHT over-expression on synaptosomal CHT-mediated choline uptake. Monitoring T lymphocyte CHT protein and activity may be useful for estimating human CNS cholinergic capacity and for testing hypotheses concerning the contribution of CHT and, more generally, ACh signaling in cognition, neuroinflammation and disease.
Precious metal recovery from secondary sources has received significant attention due to the reduced availability of precious metals from conventional sources. Herein, chitosan (CHT) was modified via cross-linking with glutaraldehyde (glu) to yield CHT-glu adsorbents with improved physicochemical and adsorption properties with precious metal ions (Au(III) and Pd(II)). CHT-glu adsorbents were prepared at variable glu ratios and characterized via complementary spectral (IR, C solids NMR, XPS) and thermogravimetry methods.
A novel bionanocomposite of grafted chitosan-phthalic anhydride/CoO nanoparticles (CHT-PHT/CoO) was synthesized and used for the elimination of brilliant green (BG) dye from aquatic systems. The CHT-PHT/CoO material underwent several instrumental characterizations including, XRD, BET, FTIR, FESEM-EDX, and pH examinations. The impact of the key uptake factors, namely A: CHT-PHT/CoO dose, B: starting solution pH, and C: contact duration, on the effectiveness of BG removal, was mathematically optimized using the response surface methodology (RSM).
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research Sciences Center Institute, Bushehr University of Medical Sciences, Bushehr, Iran.
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