Base lesions in DNA can stall the replication machinery or induce mutations if bypassed. Consequently, lesions must be repaired before replication or in a post-replicative process to maintain genomic stability. Base excision repair (BER) is the main pathway for repair of base lesions and is known to be associated with DNA replication, but how BER is organized during replication is unclear. Here we coupled the iPOND (isolation of proteins on nascent DNA) technique with targeted mass-spectrometry analysis, which enabled us to detect all proteins required for BER on nascent DNA and to monitor their spatiotemporal orchestration at replication forks. We demonstrate that XRCC1 and other BER/single-strand break repair (SSBR) proteins are enriched in replisomes in unstressed cells, supporting a cellular capacity of post-replicative BER/SSBR. Importantly, we identify for the first time the DNA glycosylases MYH, UNG2, MPG, NTH1, NEIL1, 2 and 3 on nascent DNA. Our findings suggest that a broad spectrum of DNA base lesions are recognized and repaired by BER in a post-replicative process.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737410 | PMC |
| http://dx.doi.org/10.1093/nar/gkx476 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Clinical and magnetic resonance imaging findings of presumed limber tail syndrome in a cat.
Can Vet J
January 2025
Neurology Unit, Centre Hospitalier Vétérinaire Anicura Pommery, 226 Boulevard Pommery, 51100 Reims, France.
A 4-year-old neutered female domestic shorthair indoor cat was presented with sudden onset of abnormal and painful tail-carriage. The tail was held horizontally for the first centimeters from the base and a flaccid paralysis was evident more distally. Magnetic resonance imaging of the lumbosacral spine and tail revealed a lesion located in the right and muscles along the 1st coccygeal vertebrae.
View Article and Find Full Text PDFEnhanced immunogenicity of a BoHV-1 gG-/tk- vaccine.
Vaccine
January 2025
National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China; Key Laboratory of development of veterinary diagnostic products, Ministry of Agriculture and Rural Affair, Wuhan 430070, China. Electronic address:
Bovine herpesvirus type 1 (BoHV-1) is a widespread respiratory infection that significantly impacts cattle health worldwide. To address this issue in China, we previously developed a novel double gene-deleted vaccine targeting gG and tk. In this study, we further evaluated the efficacy of this vaccine by challenging vaccinated cattle with a prevalent wild-type BoHV-1 strain and comparing its effectiveness against a commercially available inactivated BoHV-1 vaccine.
View Article and Find Full Text PDFDesign and in vitro anticancer assessment of a click chemistry-derived dinuclear copper artificial metallo-nuclease.
Nucleic Acids Res
January 2025
School of Chemical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.
Copper compounds with artificial metallo-nuclease (AMN) activity are mechanistically unique compared to established metallodrugs. Here, we describe the development of a new dinuclear copper AMN, Cu2-BPL-C6 (BPL-C6 = bis-1,10-phenanthroline-carbon-6), prepared using click chemistry that demonstrates site-specific DNA recognition with low micromolar cleavage activity. The BPL-C6 ligand was designed to force two redox-active copper centres-central for enhancing AMN activity-to bind DNA, via two phenanthroline ligands separated by an aliphatic linker.
View Article and Find Full Text PDFUse of "enhanced contact endoscopy for pituitary surgery" in a collision sellar tumor (papillary craniopharyngioma + non-functional pituitary adenoma): Representative case illustration and two-dimensional operative video.
Surg Neurol Int
December 2024
Center for Skull Base and Minimally Invasive Neurosurgery, Hospital Angeles Pedregal, Centro de Especialidades Quirúrgicas, Mexico City, Mexico.
Background: Collision sellar tumors are rare disease entities. Less than 30 cases have been reported in the literature in the last 20 years. We present the case of one patient diagnosed with a collision sellar tumor and describe the use of Enhanced Contact Endoscopy for pituitary gland and tumoral identification not previously described in the literature.
View Article and Find Full Text PDFSemi-sitting position and retrosigmoid approach for a large petroclival meningioma resection: 3-dimensional operative video.
Surg Neurol Int
December 2024
Department of Neurosurgery, Padilla Hospital of Tucuman, San Miguel de Tucuman, Argentina.
Background: Petroclival meningiomas are still a neurosurgical challenge due to their proximity to cranial nerves and cerebral vasculature along the surgical corridor. The usual extension of large petroclival meningiomas is along the posterior fossa, frequently compromising and displacing adjunct cranial nerves such as the sixth and seventh-eight cranial nerve complex with brainstem compression, causing progressive neurological deficit and severe headache. The goal of sizeable petroclival meningioma surgery treatment is a maximal resection with preservation of neurological function.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!