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Rheological comparison of sputum and reconstituted airway epithelium mucus.
Sci Rep
December 2024
Rheonova, 1 Allee de Certéze, 38610, Gières, France.
Pulmonary mucus serves as a crucial protective barrier in the respiratory tract, defending against pathogens and contributing to effective clearance mechanisms. In Muco Obstructive Pulmonary Diseases (MOPD), abnormal rheological properties lead to highly viscous mucus, fostering chronic infections and exacerbations. While prior research has linked mucus viscoelasticity to its mucin content, the variability in MOPD patients implies the involvement of other factors.
View Article and Find Full Text PDFActivated/Cycling Treg Deficiency and Mitochondrial Alterations in Immunological Non-Responders to Antiretroviral Therapy.
Front Biosci (Landmark Ed)
December 2024
Pathology Advanced Translational Research Unit, Department of Pathology & Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.
Background: Regulatory T-cells (Tregs) play a crucial role in maintaining immune homeostasis, but their dynamics are altered in a subset of people living with Human Immunodeficiency Virus (HIV) known as immunological non-responders (INRs). INRs fail to reconstitute CD4 T-cell counts despite viral suppression. This study aimed to examine Treg dysregulation in INRs, comparing them to immunological responders (IRs) and healthy controls (HCs).
View Article and Find Full Text PDFCladribine Use in Relapsing Multiple Sclerosis After 8-10 Years: Two Case Reports of Patients From the ORACLE-MS Study.
Cureus
November 2024
Neurology, Multiple Sclerosis Unit, University Hospital Nuestra Señora de la Candelaria, Santa Cruz de Tenerife, ESP.
Cladribine is an immune reconstitution therapy for multiple sclerosis (MS) that selectively produces long-term reductions in highly pathological memory B cells, with temporary reductions in other B- and T-cell subsets, thereby restoring immune function close to baseline levels in the short term. Here, we describe two cases of relapsing MS (RMS) treated with a second course of cladribine. Both patients were initially diagnosed with clinically isolated syndrome and later enrolled in the ORACLE-MS and CLASSIC-MS studies.
View Article and Find Full Text PDFEnhancing Viability of BG24 Through Optimized Spray Drying: Insights Into Process Parameters, Carrier Agents, Comparative Analysis With Freeze Drying, and Storage Condition Influences.
Food Sci Nutr
December 2024
Department of Food Engineering, Faculty of Engineering Ege University İzmir Turkey.
This study investigated the survival dynamics of BG24, a probiotic strain, within reconstituted skim milk (RSM) and yeast extract (YE) matrices during the spray-drying (SD) process, encompassing of inlet/outlet air temperatures. Notably, optimum SD parameters were found to be an inlet air temperature of 150°C and outlet air temperature of 83°C, that achieving high viability (92.23%), and reducing both moisture content (MC) (3.
View Article and Find Full Text PDFPurification and Reconstitution of Ilyobacter tartaricus ATP Synthase.
Methods Mol Biol
December 2024
Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany.
F-type Adenosine triphosphate (ATP) synthase is a membrane-bound macromolecular complex, which is responsible for the synthesis of ATP, the universal energy source in living cells. This enzyme uses the proton- or sodium-motive force to power ATP synthesis by a unique rotary mechanism and can also operate in reverse, ATP hydrolysis, to generate ion gradients across membranes. The FF-ATP synthases from bacteria consist of eight different structural subunits, forming a complex of ~550 kDa in size.
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