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Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device. | LitMetric

AI Article Synopsis

  • Creating vascular networks is essential for effective tissue engineering, but long-term culture remains a challenge without proper blood flow.
  • A new method using a microfluidic device to form a 3D cellular spheroid with an active vascular network was developed, allowing human lung fibroblasts and endothelial cells to interact.
  • This innovative design enables the vascular network to deliver biological substances within the spheroid, mimicking tissue density and potentially improving long-term tissue culture.

Article Abstract

Creating vascular networks in tissues is crucial for tissue engineering. Although recent studies have demonstrated the formation of vessel-like structures in a tissue model, long-term culture is still challenging due to the lack of active perfusion in vascular networks. Here, we present a method to create a three-dimensional cellular spheroid with a perfusable vascular network in a microfluidic device. By the definition of the cellular interaction between human lung fibroblasts (hLFs) in a spheroid and human umbilical vein endothelial cells (HUVECs) in microchannels, angiogenic sprouts were induced from microchannels toward the spheroid; the sprouts reached the vessel-like structures in a spheroid to form a continuous lumen. We demonstrated that the vascular network could administer biological substances to the interior of the spheroid. As cell density in the spheroid is similar to that of a tissue, the perfusable vasculature model opens up new possibilities for a long-term tissue culture in vitro.

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Source
http://dx.doi.org/10.1039/c7ib00024cDOI Listing

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