AI Article Synopsis
- Cytoprotection of living cells is crucial for applications in cell therapy, sensors, regenerative medicine, and food industry, focusing on maintaining cell viability under various conditions.
- A two-step encapsulation process creates a uniform shell made of organic and inorganic materials, providing enhanced resistance to factors like lytic enzymes, desiccation, and UV-C light.
- The study reveals that the encapsulated yeast not only benefits from the protective shell but also exhibits biochemical responses that enhance UV-C tolerance, making this approach useful for both cytoprotection and cellular behavior research.
Article Abstract
The cytoprotection of individual living cells under and daily-life conditions is a prerequisite for various cell-based applications including cell therapy, cell-based sensors, regenerative medicine, and even the food industry. In this work, we use a cytocompatible two-step process to encapsulate in a highly uniform nanometric (<100 nm) shell composed of organic poly(norepinephrine) and inorganic silica layers. The resulting cell-in-shell structure acquires multiple resistance against lytic enzyme, desiccation, and UV-C irradiation. In addition to the UV-C filtering effect of the double-layered shell, the biochemical responses of the encapsulated yeast are suggested to contribute to the observed UV-C tolerance. This work offers a chemical tool for cytoprotecting individual living cells under multiple stresses and also for studying biochemical behavior at the cellular level.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433039 | PMC |
| http://dx.doi.org/10.1039/c4sc02789b | DOI Listing |
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