We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.
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Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions.
Microb Cell Fact
October 2019
Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège - Gembloux Agro-Bio Tech, Gembloux, Belgium.
Background: The oleaginous yeast Yarrowia lipolytica is increasingly used as an alternative cell factory for the production of recombinant proteins. Recently, regulated promoters from genes EYK1 and EYD1, encoding an erythrulose kinase and an erythritol dehydrogenase, respectively, have been identified and characterized in this yeast. Hybrid promoters up-regulated by polyols such as erythritol and erythrulose have been developed based on tandem copies of upstream activating sequences from EYK1 (UAS1) and XPR2 (encoding extracellular protease, UAS1) promoters.
View Article and Find Full Text PDFIdentification and characterization of EYD1, encoding an erythritol dehydrogenase in Yarrowia lipolytica and its application to bioconvert erythritol into erythrulose.
Bioresour Technol
January 2018
Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège - Gembloux Agro-Bio Tech, Belgium. Electronic address:
In this study, gene YALI0F01650g has been isolated and characterized. Several experimental evidences suggest that the identified gene, renamed EYD1, encodes an erythritol dehydrogenase. An efficient bioreactor process for the bioconversion of erythritol into erythrulose was also developed.
View Article and Find Full Text PDFIdentification and characterization of EYK1, a key gene for erythritol catabolism in Yarrowia lipolytica.
Appl Microbiol Biotechnol
September 2017
Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège-Gembloux Agro-Bio Tech, Gembloux, Belgium.
Erythritol is a four-carbon sugar alcohol synthesized by osmophilic yeasts, such as Yarrowia lipolytica, in response to osmotic stress. This metabolite has application as food additive due to its sweetening properties. Although Y.
View Article and Find Full Text PDFEYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica.
J Microbiol Methods
August 2017
Microbial Processes and Interactions, TERRA Teaching and Research Center, University of Liège - Gembloux AgroBio Tech, Gembloux, Belgium. Electronic address:
We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation.
View Article and Find Full Text PDFEnhancing erythritol productivity in Yarrowia lipolytica using metabolic engineering.
Metab Eng
July 2017
Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège - Gembloux Agro-Bio Tech, Belgium. Electronic address:
Erythritol (1,2,3,4-butanetetrol) is a four-carbon sugar alcohol with sweetening properties that is used by the agrofood industry as a food additive. In this study, we demonstrated that metabolic engineering can be used to improve the production of erythritol from glycerol in the yeast Yarrowia lipolytica. The best results were obtained using a mutant that overexpressed GUT1 and TKL1, which encode a glycerol kinase and a transketolase, respectively, and in which EYK1, which encodes erythrulose kinase, was disrupted; the latter enzyme is involved in an early step of erythritol catabolism.
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