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| http://dx.doi.org/10.3324/haematol.2016.162966 | DOI Listing |
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The GWAS SNP rs80207740 modulates erythrocyte traits via allele-specific binding of IKZF1 and targeting XPO7 gene.
FASEB J
May 2024
State Key Laboratory of Complex, Severe, and Rare Diseases, Haihe Laboratory of Cell Ecosystem, Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
Genome-wide association studies have identified many single nucleotide polymorphisms (SNPs) associated with erythrocyte traits. However, the functional variants and their working mechanisms remain largely unknown. Here, we reported that the SNP of rs80207740, which was associated with red blood cell (RBC) volume and hemoglobin content across populations, conferred enhancer activity to XPO7 gene via allele-differentially binding to Ikaros family zinc finger 1 (IKZF1).
View Article and Find Full Text PDFA novel -regulatory element regulates αD and αA-globin gene expression in chicken erythroid cells.
Front Genet
April 2024
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México City, Mexico.
Background: -regulatory elements (CREs) play crucial roles in regulating gene expression during erythroid cell differentiation. Genome-wide erythroid-specific CREs have not been characterized in chicken erythroid cells, which is an organism model used to study epigenetic regulation during erythropoiesis.
Methods: Analysis of public genome-wide accessibility (ATAC-seq) maps, along with transcription factor (TF) motif analysis, CTCF, and RNA Pol II occupancy, as well as transcriptome analysis in fibroblasts and erythroid HD3 cells, were used to characterize erythroid-specific CREs.
A comparative study of two routinely used protocols for ex vivo erythroid differentiation.
Blood Cells Mol Dis
May 2024
Etablissement Français du Sang PACA-Corse, Aix Marseille University, CNRS, ADES UMR 7268, 13005 Marseille, France; Laboratoire d'Excellence GR-Ex, 75000 Paris, France. Electronic address:
Background: Erythropoiesis is a complex developmental process in which a hematopoietic stem cell undergoes serial divisions and differentiates through well-defined stages to give rise to red blood cells. Over the last decades, several protocols have been developed to perform ex vivo erythroid differentiation, allowing investigation into erythropoiesis and red cell production in health and disease.
Results: In the current study, we compared the two commonly used protocols by assessing the differentiation kinetics, synchronisation, and cellular yield, using molecular and cellular approaches.
Genome-wide transcription factor-binding maps reveal cell-specific changes in the regulatory architecture of human HSPCs.
Blood
October 2023
School of Clinical Medicine, University of New South Wales, Sydney, Australia.
Article Synopsis
- Hematopoietic stem and progenitor cells (HSPCs) require a specific combination of seven transcription factors (TFs) to manage their differentiation into various blood cell types, but their precise roles are still unclear.
- The study used advanced techniques to analyze chromatin interactions and TF binding in different HSPC subtypes, revealing that TF activity and enhancer-promoter interactions differ significantly among these cell types, which correlates with how genes are expressed.
- Findings indicate that certain TF combinations are linked to specific blood cell lineages, and these combinations may also prime regulatory regions for future binding by lineage-specific TFs, providing insights into both normal blood cell formation and potential disruption in leukemia.
The role of GATA switch in benzene metabolite hydroquinone inhibiting erythroid differentiation in K562 cells.
Arch Toxicol
August 2023
School of Biological Science and Medical Engineering, Beihang University, 37 Xueyuan Road, Beijing, 100191, China.
The phenolic metabolite of benzene, hydroquinone (HQ), has potential risks for hematological disorders and hematotoxicity in humans. Previous studies have revealed that reactive oxygen species, DNA methylation, and histone acetylation participate in benzene metabolites inhibiting erythroid differentiation in hemin-induced K562 cells. GATA1 and GATA2 are crucial erythroid-specific transcription factors that exhibit dynamic expression patterns during erythroid differentiation.
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