The effect of NGF from bovine seminal plasma on the adenylate cyclase system of pheochromocytoma PC12 cell line was studied. It was shown that the elevation of the intracellular level of cAMP caused the NGF-like morphological differentiation of PC12 cells cultured in the serum-free medium. This effect was very transitory because of the compensatory action of phosphodiesterase of cAMP, the biosynthesis of which was induced by the elevated level of intracellular cAMP. Inhibition of the adenylate cyclase activity in the membrane preparation of PC12 cells was observed. This inhibition can be a result of a decrease in the level of endogenous ADP-ribosylation which was detected after incubation of cells with NGF. The parameters of this process were investigated. It was demonstrated that morphological differentiation of PC12 cells can be induced by serotonin (neurotransmitter), L-carnosine (dipeptide) and retinoic acid (vitamin A derivative). The possible mechanisms of action of these substances were suggested. It was shown that serotonin inhibited adenylate cyclase of PC12 cells, whereas the combined action of NGF and serotonin led to the activation of the enzyme. The hypothetic mechanism of this process was proposed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0065-2571(88)90026-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In situ biosensing for cell viability and drug evaluation in 3D extracellular matrix cultures: Applications in cytoprotection of oxidative stress injury.
Talanta
January 2025
Department of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350122, China. Electronic address:
The rise of extracellular matrix (ECM)-supported three-dimensional (3D) cell culture systems which bridge the gap between in vitro culture and in vivo living tissue for pharmacological models has increased the need for simple and robust cell viability assays. This study presents the development of an effective biosensing assay for in situ monitoring of the catecholamine neurotransmitter exocytosis levels for cell viability assessment within complicated cell-encapsulated hydrogel milieu. Firstly, the biosensing assay demonstrated the distinction among four pheochromocytoma (PC12) cell lines with varying degrees of differentiation and the discrepancy in cellular neurosecretory capacity between two-dimensional (2D) monolayer and 3D agarose hydrogel culture conditions, accompanied by morphological distinctions.
View Article and Find Full Text PDFE-jet 3D printed aligned nerve guidance conduits incorporated with decellularized extracellular matrix hydrogel encapsulating extracellular vesicles for peripheral nerve repair.
Acta Biomater
January 2025
Central laboratory of Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China. Electronic address:
Peripheral nerve injury (PNI) as a common clinical issue that presents significant challenges for repair. Factors such as donor site morbidity from autologous transplantation, slow recovery of long-distance nerve damage, and deficiencies in local cytokines and extracellular matrix contribute to the complexity of effective PNI treatment. It is extremely urgent to develop functional nerve guidance conduits (NGCs) as substitutes for nerve autografts.
View Article and Find Full Text PDFBackground: Fibroblast growth factor 21 (FGF21) and Methyltransferase-like 14 (METTL14) have been identified to be involved in spinal cord injury (SCI). However, whether FGF21 functioned in SCI via METTL14-induced N6-methyladenosine (m6A) modification remains unclear.
Materials And Methods: PC12 cells were exposed to lipopolysaccharide (LPS) in vitro.
A polysaccharide from Morchella esculenta mycelia: Structural characterization and protective effect on antioxidant stress on PC12 cells against HO-induced oxidative damage.
Int J Biol Macromol
January 2025
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; State Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China. Electronic address:
Morchella esculenta (L.) Pers. is considered a precious edible and medicinal fungus due to its strict growth environment requirements, difficult to cultivate, resulted in expensive in the market.
View Article and Find Full Text PDFBackground: Prostaglandin E (PGE) in the rostral ventrolateral medulla (RVLM) has been recognized as a pivotal pressor substance in hypertension, yet understanding of its effects and origins in the RVLM remains largely elusive. This study aimed to elucidate the pivotal enzymes and molecular mechanisms underlying PGE synthesis induced by central Ang II (angiotensin II) and its implications in the heightened oxidative stress and sympathetic outflow in hypertension.
Methods And Results: RVLM microinjections of PGE and Tempol were administered in Wistar-Kyoto rats.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!