Objective: Monitoring hemodynamic and vascular changes in the acute stages of mouse stroke models is invaluable in studying ischemic stroke pathophysiology. However, there lacks a tool to simultaneously and dynamically investigate these changes.
Methods: We integrated laser speckle imaging (LSI) and visible-light optical coherence tomography (Vis-OCT) to reveal dynamic vascular responses in acute stages in the distal middle cerebral artery occlusion (dMCAO) model in rodents. LSI provides full-field, real-time imaging to guide Vis-OCT imaging and monitor the dynamic cerebral blood flow (CBF). Vis-OCT offers depth-resolved angiography and oxygen saturation (sO2) measurements.
Results: Our results showed detailed CBF and vasculature changes before, during, and after dMCAO. After dMCAO, we observed insignificant sO2 variation in arteries and arterioles and location-dependent sO2 drop in veins and venules. We observed that higher branch-order veins had larger drops in sO2 at the reperfusion stage after dMCAO.
Conclusion: This work suggests that integrated LSI and Vis-OCT is a promising tool for investigating ischemic stroke in mouse models.
Significance: For the first time, LSI and Vis-OCT are integrated to investigate ischemic strokes in rodent models.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700853 | PMC |
| http://dx.doi.org/10.1109/TBME.2017.2706976 | DOI Listing |
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