Neutralization of Inflammation by Inhibiting and Secretory Phospholipase A by Ethanol Extract of L.

Background: Inflammation is a normal and necessary prerequisite to healing of the injured tissues. Inflammation contributes to all disease process including immunity, vascular pathology, trauma, sepsis, chemical, and metabolic injuries. The secretory phospholipase A (sPLA) is a key enzyme in the production of pro-inflammatory mediators in chronic inflammatory disorders such as rheumatoid arthritis, coronary heart disease, diabetes, and asthma. The sPLA also contribute to neuroinflammatory disorders such as Parkinson's, Alzheimer's, and Crohn's disease.

Aims: The present study aims to investigate the inhibition of human sPLA by a popular medicinal herb Linn. as a function of anti-inflammatory activity.

Materials And Methods: The aqueous and different organic solvents extracts of were prepared and evaluated for human synovial fluid, human pleural fluid, as well as and venom sPLA enzyme inhibition.

Results: Among the extracts, the ethanol extract of (EEBD) showed the highest sPLA inhibition and IC values ranging from 17.8 to 27.5 μg. Further, antioxidant and lipid peroxidation activities of extract were checked using 2,2-diphenyl-1-picrylhydrazyl radical, thiobarbituric acid, and rat liver homogenate. The antioxidant activity of EEBD was more or less directly proportional to sPLA inhibition. Eventually, the extract was subjected to neutralize sPLA-induced mouse paw edema and indirect hemolytic activity. The EEBD showed similar potency in both the cases.

Conclusions: The findings suggest that the bioactive molecule/s from the EEBD is/are potentially responsible for the observed and sPLA inhibition and antioxidant activity.

Summary: The present study aims to investigate the inhibition of human sPLA by a popular medicinal herb Linn. as a function of anti inflammatory activity. EEBD: Ethanolic extract of boerhaavia diffusa, sPLA: Secretory phospholipase A, HSF: Human synovial fluid, HPF: Human pleural fluid, VRV-PLA2-V: phospholipase A2, NN-PLA-I: phospholipase A.