Lucigenin (Bis-N-methylacrylium nitrate) reacts with H2O2 to produce ground state and excited state monomer. The excited state monomer decays with the emission of light. When lucigenin is added to reaction mixtures containing vitamin-K-dependent carboxylase, light emission is produced which is inversely proportional to enzyme concentration and co-inhibited with inhibitors of vitamin-K-dependent carboxylase.
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