Generation of Newly Discovered Resistance Gene Knockout in Using the CRISPR/Cas9 System.

The gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce gene knockout in . Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the gene into microbial populations.