A convenient method for the preparation of oligonucleotides containing internally-attached galactose and triantennary galactose units has been developed based on click chemistry between 2'--alkyne 2'-amino-LNA nucleosides and azido-functionalized galactosyl building blocks. The synthesized oligonucleotides show excellent binding affinity and selectivity towards complementary DNA/RNA strands with an increase in the melting temperature of up to +23.5 °C for triply-modified variants.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153924 | PMC |
| http://dx.doi.org/10.3390/molecules22050852 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural analysis and immunological activity of a novel low molecular weight neutral polysaccharide isolated from Hemerocallis citrina Borani.
Food Chem
December 2024
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:
A novel neutral water-soluble polysaccharide (HCBP1-1) was isolated and purified from Hemerocallis citrina Borani by column chromatography. The fine structure of HCBP1-1 was determined by a series of physical and chemical means. HCBP1-1 was a homogeneous low molecular weight polysaccharide of 7.
View Article and Find Full Text PDFMethane production related to microbiota in dairy cattle feces.
Environ Res
December 2024
State Key Laboratory of Multiphase Flow in Power Engineering, Xi'an Jiaotong University, Xi'an, 710049, Shaanxi, China.
Methane (CH) emission from livestock feces, led by ruminants, shows a profound impact on global warming. Despite this, we have almost no information on the syntrophy of the intact microbiome metabolisms, from carbohydrates to the one-carbon units, covering multiple stages of ruminant development. In this study, syntrophic effects of polysaccharide degradation and acetate-producing bacteria, and methanogenic archaea were revealed through metagenome-assembled genomes from water saturated dairy cattle feces.
View Article and Find Full Text PDFBiochar promotes the dissolution of inorganic inactive phosphorus by mediating the bacterial community during corn stover and cattle manure composting.
Chemosphere
December 2024
College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing 163319, China. Electronic address:
Phosphorus (P) is a macroelement primarily found in insoluble forms in nature. Enhancing the effectiveness of P is crucial for sustainable agricultural development and ecosystems. The research employed a combination of sequential extraction methods, high-throughput sequencing techniques, microbial culturing, and ecological network analysis of bacterial communities, along with module comparison, to explore the dynamics of different P fractions in calcareous soils.
View Article and Find Full Text PDFEffects of various supplemental levels of multi-enzyme complex on amino acid profiles in egg yolk, antioxidant capacity, cecal microbial community and metabolites of laying hens.
Front Microbiol
November 2024
Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, China.
This study aimed to investigate the effects of multi-enzyme (alkaline protease, xylanase, glucanase, β-mannanase, cellulase, acid protease, glucoamylase, and α-galactosidase) on antioxidant capacity, egg quality, amino acid profiles in yolk, cecal microflora and metabolites in laying hens. A total of 384 Jingfen No.6 laying hens aged 65 weeks were randomly divided into 4 treatments groups (6 replicates per group) and fed diets containing 0, 150, 300, or 600 mg kg multi-enzyme over an 8-week feeding duration.
View Article and Find Full Text PDFPhotoclick surface modification of MOF-808 for galactose-mediated targeted chemotherapy.
J Colloid Interface Sci
March 2025
School of Chemistry, University of Glasgow, Glasgow G12 8QQ, UK. Electronic address:
Controllable surface modification of nanoparticulate drug delivery vectors is key to enhancing specific desirable properties such as colloidal stability, targeting, and stimuli-responsive cargo release. Metal-organic frameworks (MOFs) have been proposed as potential delivery devices, with surface modification achieved by various bioconjugate "click" reactions, including copper-catalysed and strain-promoted azide-alkyne cycloaddition. Herein, we show that photo-induced nitrile imine-mediated tetrazole-ene cycloaddition (NITEC) can be used to surface-modify tetrazole-appended Zr MOFs with maleimides, and vice versa, with the extent of this traceless surface functionalisation controlled by the length of photoirradiation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!