Adoptive T-cell immunotherapy is a promising approach to cancer therapy. Stem cell memory T (T) cells have been proposed as a class of long-lived and highly proliferative memory T cells. CD8 T cells can be generated in vitro from naive CD8 T cells via Wnt signalling; however, methods do not yet exist for inducing T cells from activated or memory T cells. Here, we show a strategy for generating T-like cells in vitro (iT cells) from activated CD4 and CD8 T cells in mice and humans by coculturing with stromal cells that express a Notch ligand. iT cells lose PD-1 and CTLA-4 expression, and produce a large number of tumour-specific effector cells after restimulation. This method could therefore be used to generate antigen-specific effector T cells for adoptive immunotherapy.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458121 | PMC |
| http://dx.doi.org/10.1038/ncomms15338 | DOI Listing |
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